Klf4 chip-seq
WebApr 1, 2024 · Results of in vivo KLF4 ChIP-Seq analyses, and studies in cultured SMC treated with cholesterol identified > 800 KLF4 target genes including many that regulate pro-inflammatory responses of SMC. Results indicate that the contribution of SMCs within atherosclerotic plaques has been greatly underestimated, and that KLF4-dependent … WebMay 15, 2024 · GEO help: Mouse over screen elements for information. RNA-seq, ChIP seq and Whole genome bisulfite seq for KLF4-mCpG binding in GBM cells. We engineered two doxycycline-inducible U87 cell lines to express KLF4 wild type and mutant (R458A), and examined KLF4 binding, gene regulation and whole genome bisulfite seq in U87 cells.
Klf4 chip-seq
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WebKruppel-like factor 4 (KLF4; gut-enriched Krüppel-like factor or GKLF) is a member of the KLF family of zinc finger transcription factors, which belongs to the relatively large family of … WebApr 11, 2024 · Furthermore, the authors identified the Dlx3, Klf4, ... ChiP-seq analyses of the Dlx3 genome-wide binding showed predominance of the Dlx3 peaks in intergenic regions (47%) over the proximal promoters (15%), suggesting a role for Dlx3 in long-range chromatin interactions. Importantly, further correlation of the peaks enriched for both Dlx3 and ...
WebApr 14, 2024 · The ChIP-seq data for transcriptional regulators were downloaded and are listed in Supplementary Data 7. RNA-seq data for Tbx3 KD, Ctr9 KD and Pcgf6 KD mESCs have been deposited in the GEO under ... WebGO enrichment analysis of the RNA-seq and ChIP-seq results showed that genes regulated by TCF21 were associated with the categories ‘ECM organisation’ and ‘ECM’ (figure 3C, D), ... Klf4-Dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nat Med 2024; 23:1176–90. 10.1038/nm.4400 [PMC free ...
WebMay 12, 2011 · ChIP-seq analyses of RAD21 reveal an ESC specific cohesin binding pattern that is characterized by CTCF independent co-localization of cohesin with pluripotency related transcription factors Oct4, Nanog, Sox2, Esrrb and Klf4. WebJul 17, 2024 · Remarkably, we provide evidence that Klf4 and Oct4 control nearly opposite patterns of gene expression in SMC and based on in vivo ChIP-seq analyses have …
WebDec 22, 2009 · The recent development of ultra-high-throughput RNA sequencing (RNA-Seq) technology holds the promise to provide more accurate gene expression measurements (1, 2).Compared with microarray, RNA-Seq is able to measure absolute concentration of transcripts ().Meanwhile, chromatin immunoprecipitation (ChIP) coupled with microarray …
WebJul 10, 2024 · KLF4 ChIP-Seq. We demonstrated, using KLF gain-and-loss-of-function experiments, that KLF is required to mediate chromatin accessibility changes at proximal and distal sites, adding to the known role for KLF in transcriptional activation of flow responsive genes. psyche\u0027s apWebJan 19, 2024 · Cooperative binding of Oct4, Sox2, and Klf4 with stage-specific transcription factors orchestrates reprogramming [ChIP-seq] Oct4, Sox2, Klf4, and cMyc (OSKM) … psyche\u0027s aoWebFeb 3, 2015 · A genome-wide RNA-seq analysis was conducted to identify genes regulated by KLF4 in T-ALL cells. Chromatin immunoprecipitation (ChIP) PCR was used to determine direct binding sites of KLF4 in T-ALL cells. Here we reveal that KLF4 induced apoptosis through the BCL2/BCLXL pathway in human T-ALL cell lines and primary T-ALL specimens. psyche\u0027s alWebAnti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting. - Find MSDS or SDS, a COA, data sheets and more information. psyche\u0027s anWebDec 11, 2024 · ChIP-seq data and analysis. For ChIP-seq analyses, 1x10 7 cells of the Akt transformed prostate stem cell line with endogenous Klf4 were fixed in PBS containing 1% of formaldehyde for 8 min at room temperature on a rotating platform. The reaction was quenched with 0.125M glycine for 5 min. horwath mak business consultingWebJun 5, 2014 · Analysis of Tcf3 genomic binding sites through chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq) reveals that Gsk3 signaling inhibition alleviates Tcf3-mediated transcriptional repression of … horwath law firmWebJul 3, 2013 · To further explore the molecular mechanisms of Klf4’s role in mediating long-range interactions, we focused on the potential interacting partners of Klf4 in PSCs. Previous ChIP-seq data have demonstrated that Klf4 colocalizes with cohesin complex (Nitzsche et al., 2011), which has been shown to be a critical player in DNA looping (Kagey et al ... psyche\u0027s art summary