WebThese DNA fragments served as the initiator of the polymerization catalyzed by TdTase, and this constitutes a second stage of the process. In the third stage, a solution containing TdTase and a mononucleotide (dTTP proved to be the most efficient among those studied) was applied to the sample. The enzyme caused the addition of more T bases to ... WebMay 15, 2024 · TdTase is a DNA polymerase that can incorporate nucleotides into the 3′-OH termini of single-stranded DNA (ssDNA) without needing a DNA or RNA template ( Liu et …
Schematic illustration of TdT activity detection based on in- site ...
WebJan 15, 2024 · A new strategy based on enzymatically engineered primer extension poly-thymine (EPEPT) and nanomaterials in situ generation technology is reported for direct detection of microRNA (miRNA) in a fluorescence turn-on format using the sequential and complementary reactions catalyzed by Klenow Fragment exo-(KFexo-) and terminal … WebWhat does TDTASE stand for in Biology? Get the top TDTASE abbreviation related to Biology. Suggest. TDTASE Biology Abbreviation. What is TDTASE meaning in Biology? … most complex border in the world
Electrochemical DNA biosensor based on grafting-to …
WebThe activity of terminal deoxynucleotidyl transferase (TdTase) is a biomarker for routine diagnosis of acute leukemia. A method has been developed for the determination of … WebDec 15, 2024 · However, this method, in which the background is significantly affected by the amount of TdTase, suffers from being easy to produce false positive result and poor stability. Herein, we firstly present a novel electrochemical DNA biosensor based on grafting-to mode of TdTase-mediated extension, in which DNA targets are polymerized in homogeneous ... WebNov 25, 2024 · The TdTase-mediated extension reaction was performed in a mixture solution for 30 min at 37 °C (20 μL volume of mixed solution containing 10 μL AuNPs-Apt1, 4.0 μL reaction buffer, 2.0 μL 10 mM dATP (or) dNTPs, 1.0 μL TdTase, 3.0 μL ddH2O, respectively). After that, the products, Au-Apt1-polyA or AuNPs-Apt1-random bases … most complex internet network design